Description
Priciple of the assay: This assay employs a two-site sandwich ELISA to quantitative F2RL3 in Human serum, plasma, tissue homogenates. An antibody specific for F2RL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F2RL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for F2RL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F2RL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protease-activated receptor 4 (PAR-4), also known as coagulation factor II (thrombin) receptor-like 3, is a protein that in humans is encoded by the F2RL3 gene. Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosinenucleotide- binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. Protease-activated receptors 1 (PAR1), 2 (PAR2), and 3 (PAR3) are members of a unique G protein-coupled receptor family. They are characterized by a tethered peptide ligand at the extracellular amino terminus that is generated by minor proteolysis.By fluorescence in situ hybridization, Xu et al. (1998) mapped the PAR4 gene to 19p12